2 Aims and Objectives

Stem cell and tissue engineering technologies allow for the potential treatment of heart failure with engineered tissue construtcs — the EHMs. We thus explored RNA-Seq data collected at two stages, as CMs and as EHMs, with the following aims and objectives:

1. To establish a reusable workflow to analyse the sequenced data — from raw FASTQ files to count files.

2. To check for the potential of RNA-Seq to identify microbial contamination.

3. To explore the data in context of maturity by comparing with adult and fetal samples from publically available datasets.

4. To identify the potential sub-populations via digital deconvolution techniques using a relevant scRNA-Seq dataset.